Related Experiment Video
Updated: Mar 6, 2026
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
Published on: March 19, 2010
A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells
Hongkai Zhang1, Richard A Lerner2
1Department of Cell and Molecular Biology, The Scripps Research Institute, Mail Code: MB 10, 10550 N. Torrey Pines Rd, La Jolla, CA, 92037, USA.
Abstract:
There is a need for a general reporter system to study the interactions between a ligand and membrane protein in living cells. Here, we demonstrate a method that allows identification of undiscovered ligand partners of a membrane protein in its natural milieu. This assay will be of importance especially for multiple pass transmembrane proteins such as ion channels and GPCRs.
More Related Videos
04:47Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
Published on: May 22, 2020
09:39Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
Published on: August 2, 2024