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Control of respiration rate in non-growing cells of Paracoccus denitrificans
I Kucera1, L Lampardová, V Dadák
1Department of Biochemistry, Faculty of Science, J.E. Purkynĕ University, Brno, Czechoslovakia.
The Biochemical Journal
|September 15, 1987
Abstract:
By means of fluorimetric measurement and by direct determination of intracellular NAD+ and NADH contents, it was proved that the respiration rate of Paracoccus denitrificans cells utilizing glucose is limited by processes preceding NADH oxidation in the respiratory chain, so that the membrane NADH dehydrogenase is not saturated by its substrate. In the separated membrane fraction on saturation with exogenous NADH the main limiting factor is represented by NADH: ubiquinone oxidoreductase.