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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 6, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Universal Super-Resolution Multiplexing by DNA Exchange.

Florian Schueder^1,2, Maximilian T Strauss^1,2, David Hoerl³

¹Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.

Angewandte Chemie (International Ed. in English)

|March 4, 2017

Summary

This summary is machine-generated.

This study introduces a fast DNA probe exchange method for rapid, sequential multiplexing in super-resolution microscopy. This technique enhances efficiency for detecting multiple targets across various super-resolution imaging methods.

Keywords:

DNA nanotechnology SIM STED dSTORM multiplexing

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Area of Science:

Optical Microscopy
Molecular Biology
Nanotechnology

Background:

Super-resolution microscopy offers enhanced spatial resolution beyond the diffraction limit.
Multiplexed imaging in super-resolution microscopy is challenging and time-consuming.

Purpose of the Study:

To develop a straightforward and rapid sequential multiplexing approach for super-resolution microscopy.
To enable efficient detection of multiple targets using common super-resolution techniques.

Main Methods:

A sequential multiplexing strategy utilizing fast DNA probe exchange.
Assays performed on DNA origami nanostructures for quantitative assessment.
Application demonstrated by imaging multiple protein targets in fixed cells.

Main Results:

The DNA probe exchange method enables efficient and rapid multiplexed target detection.
Quantitative assessment using DNA origami confirmed labeling, imaging, and washing efficiency.
Successful imaging of multiple protein targets in fixed cells was achieved.

Conclusions:

The developed approach provides a versatile and efficient solution for multiplexing in super-resolution microscopy.
This method significantly reduces the time and complexity associated with multi-target super-resolution imaging.
Facilitates advanced biological investigations requiring simultaneous visualization of multiple molecular targets.