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Other Nuclides: 31P, 19F, 15N NMR

Other Nuclides: ³¹P, ¹⁹F, ¹⁵N NMR

Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research.
While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a...

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Related Experiment Video

Updated: Mar 6, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis

Published on: May 10, 2020

Modified Method for Trapping and Analyzing ¹⁵N in NO Released from Soils.

Ronghua Kang¹, Jan Mulder¹, Peter Dörsch¹

¹Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences , N-1432, Aas, Norway.

Analytical Chemistry

|March 4, 2017

Summary

This study presents a new method for measuring ¹⁵N in soil nitric oxide (NO). The technique effectively traps and converts low concentrations of NO for precise isotopic analysis, aiding soil nitrogen cycling research.

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Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

Area of Science:

Environmental Science
Soil Science
Isotope Geochemistry

Background:

Investigating soil nitric oxide (NO) sources is crucial for understanding nitrogen cycling.
Direct ¹⁵N isotope tracing of NO is challenging due to its reactivity and low soil-surface concentrations.
Existing wet-chemical methods are often unsuitable for the low NO concentrations found in soil environments.

Purpose of the Study:

To develop and validate a modified method for efficient trapping and conversion of low-concentration NO for ¹⁵N analysis.
To enable accurate isotopic quantification of NO derived from various soil nitrogen pools.
To improve the investigation of soil NO production pathways using ¹⁵N isotope tracing.

Main Methods:

Quantitative oxidation of NO to NO₂ using chromium trioxide (CrO₃).
Conversion of NO₂ to nitrite (NO₂^-) and nitrate (NO₃^-) in an alkaline hydrogen peroxide (H₂O₂) solution.
Reduction of NO₂^- and NO₃^- to nitrous oxide (N₂O) using a denitrifier method for ¹⁵N analysis.

Main Results:

Achieved NO trapping efficiencies exceeding 85% for 50 ppb NO in an air stream.
Demonstrated high precision: 0.29‰ (δ-values) for natural abundance NO and 0.13 atom % for labeled NO.
Successfully identified distinct ¹⁵N abundances in NO from soil amendments (¹⁵NH₄NO₃, NH₄¹⁵NO₃, Na¹⁵NO₂).

Conclusions:

The modified method provides an effective and precise approach for ¹⁵N analysis of low-concentration soil NO.
This technique is suitable for both natural abundance and ¹⁵N labeling studies in soil ecosystems.
The method facilitates the elucidation of NO sources and pathways in diverse soil environments.