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Updated: Oct 8, 2026

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
Published on: September 6, 2014
A simple and rapid method for the purification of the mitochondrial porin from mammalian tissues
V de Pinto1, G Prezioso, F Palmieri
1Department of Pharmaco-Biology, University of Bari, Italy.
Abstract:
A new, simple and rapid procedure for the purification of high amounts of mitochondrial porins from different tissues of mammalia is described. The method consists in a single step hydroxyapatite/celite chromatography of Triton X-100 solubilized mitochondrial membranes. For optimal purification several factors are critical such as the absence of salts, a low protein/detergent ratio and an exact hydroxyapatite/celite ratio of 2:1.
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