Graphite paper-based bipolar electrode electrochemiluminescence sensing platform

Area of Science:

• Electrochemistry
• Biosensing
• Materials Science

Background:

• Development of sensitive and cost-effective biosensing platforms is crucial for clinical diagnostics.
• Existing methods often face limitations in terms of cost, portability, and sensitivity.
• Disposable and wireless sensing systems offer significant advantages for point-of-care applications.

Purpose of the Study:

• To develop a novel, disposable, wireless, and low-cost electrochemiluminescence (ECL) sensing platform.
• To utilize a closed bipolar electrode (BPE) configuration based on graphite paper for enhanced sensing capabilities.
• To demonstrate the platform's efficacy in quantifying both an oxidant (hydrogen peroxide) and a biomarker (carcinoembryonic antigen, CEA).

Main Methods:

• Fabrication of a closed bipolar electrode (BPE) electrochemiluminescence (BPE-ECL) sensing platform using graphite paper.
• Quantitative analysis of hydrogen peroxide (H2O2) using platinum nanoparticles (Pt NPs) electrodeposited on the cathode.
• Quantitative analysis of carcinoembryonic antigen (CEA) utilizing chitosan-multi-walled carbon nanotubes (CS-MWCNTs) for antibody immobilization and Au@Pt nanostructures for catalysis.

Main Results:

• The BPE-ECL device achieved a wide linear range of 0.001-15 mM with a low detection limit of 0.5 µM for H2O2.
• The immunodevice demonstrated a wide linear range of 0.01-60 ng/mL with a detection limit of 5.0 pg/mL for CEA.
• The developed platform exhibited satisfactory selectivity, excellent stability, and good reproducibility.

Conclusions:

• A novel and highly sensitive BPE-ECL sensing platform based on graphite paper has been successfully developed.
• The platform demonstrates significant potential for the quantitative analysis of both small molecules and complex biomarkers in clinical settings.
• This work opens new avenues for the development of cost-effective, disposable, and wireless bioassay systems for clinical applications.