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Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Published on: March 9, 2021
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Towards understanding cellular structure biology: In-cell NMR
Safikur Rahman1, Younhwa Byun1, Md Imtaiyaz Hassan2
1Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, South Korea.
Biochimica Et Biophysica Acta. Proteins and Proteomics
|March 5, 2017
Summary
In-cell nuclear magnetic resonance (NMR) allows observing biomolecules within living cells at atomic resolution. This technique reveals crucial insights into protein structure, interactions, and dynamics in their native cellular environment.
Area of Science:
- Biophysics
- Structural Biology
- Cellular Biology
Background:
- Observing biological macromolecules functioning within living cells is a long-standing goal in biology.
- In-cell nuclear magnetic resonance (NMR) spectroscopy offers a powerful approach to study these molecules at the atomic level.
Purpose of the Study:
- To review current methodologies in in-cell NMR.
- To highlight its applications in understanding protein behavior within cells.
Main Methods:
- In-cell NMR spectroscopy is employed to analyze biomolecular structures, interactions, and dynamics.
- The technique is applicable across various cellular systems.
Main Results:
- In-cell NMR provides atomic-level insights into protein interactions, folding, stability, and post-translational modifications.
- It enables the study of intricate biological processes in their native cellular context.
Conclusions:
- In-cell NMR is an effective tool for structural analysis and studying complex biological mechanisms within cellular environments.
- This review underscores the value of in-cell NMR for advancing biological research.
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