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Updated: Mar 6, 2026

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

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Published on: January 26, 2016

SICLOPPS cyclic peptide libraries in drug discovery.

Ali Tavassoli¹

¹Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.

Current Opinion in Chemical Biology

|March 5, 2017

Summary

Genetically encoded cyclic peptide libraries offer advantages for drug discovery. The split-intein circular ligation of peptides and proteins (SICLOPPS) method enables efficient identification of peptide inhibitors for challenging targets.

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Cyclic peptides show promise for targeting difficult biological interactions, including protein-protein interactions.
Genetically encoded peptide libraries are advantageous over chemical synthesis due to accessibility and ease of hit identification.
Split-intein circular ligation of peptides and proteins (SICLOPPS) is a key method for intracellular library generation.

Purpose of the Study:

To detail and discuss the application of SICLOPPS for generating cyclic peptide libraries.
To highlight the utility of SICLOPPS in identifying inhibitors for various biological targets.

Main Methods:

Utilizing the SICLOPPS technique for the intracellular generation of cyclic peptide libraries.
Employing these libraries to screen for and identify peptide inhibitors.

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Main Results:

Demonstrated the successful deployment of SICLOPPS for library creation.
Showcased the identification of cyclic peptide inhibitors against diverse targets using this approach.

Conclusions:

SICLOPPS is a powerful and versatile method for generating genetically encoded cyclic peptide libraries.
This approach facilitates the discovery of novel cyclic peptide inhibitors for challenging therapeutic targets.