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Updated: Mar 6, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Published on: January 16, 2017
RNP transport in cell biology: the long and winding road
Carolina Eliscovich1, Robert H Singer2
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, United States; Current address: Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, United States.
Abstract:
Regulation of gene expression is key determinant to cell structure and function. RNA localization, where specific mRNAs are transported to subcellular regions and then translated, is highly conserved in eukaryotes ranging from yeast to extremely specialized and polarized cells such as neurons. Messenger RNA and associated proteins (mRNP) move from the site of transcription in the nucleus to their final destination in the cytoplasm both passively through diffusion and actively via directed transport. Dysfunction of RNA localization, transport and translation machinery can lead to pathology. Single-molecule live-cell imaging techniques have revealed unique features of this journey with unprecedented resolution. In this review, we highlight key recent findings that have been made using these approaches and possible implications for spatial control of gene function.
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