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Comparison of quantitative and qualitative tests for glucose-6-phosphate dehydrogenase deficiency in the neonatal

F Keihanian1,2, S Basirjafari1,3, B Darbandi4

  • 1Guilan University of Medical Sciences, Rasht, Iran.

International Journal of Laboratory Hematology
|March 5, 2017
PubMed

Insights

Glucose-6-phosphate dehydrogenase (G6PD) deficiency affects 6.6% of newborns in Rasht, Iran. The fluorescent spot test (FST) shows good sensitivity and specificity for screening G6PD deficiency in neonates.

Area of Science:

  • Medical Sciences
  • Pediatrics
  • Genetics

Background:

  • Glucose-6-phosphate dehydrogenase (G6PD) deficiency is common in newborns globally.
  • Various screening methods are employed for G6PD deficiency detection in neonates.
  • Understanding regional prevalence is crucial for public health initiatives.

Purpose of the Study:

  • To determine the prevalence of G6PD deficiency in newborns in Rasht, Iran.
  • To compare the efficacy of quantitative and qualitative tests for G6PD activity assessment.
  • To evaluate the diagnostic performance of the fluorescent spot test (FST).

Main Methods:

  • A cross-sectional, prospective study involving 1474 newborns in Rasht, Iran.
  • G6PD activity was measured using both kinetic assay (quantitative) and fluorescent spot test (qualitative).
  • Sensitivity, specificity, and predictive values of the FST were calculated.

Main Results:

  • A prevalence of 6.6% for G6PD deficiency was observed among the studied newborns.
  • Significant differences in mean G6PD levels were found between male and female newborns (P = 0.0001).
  • A strong correlation was established between FST results and quantitative G6PD activity measurements (P < 0.0001).

Conclusions:

  • The fluorescent spot test (FST) demonstrates acceptable sensitivity and specificity for G6PD deficiency screening in newborns.
  • While FST is a useful screening tool, its diagnostic efficiency may be limited in certain situations.
  • The study highlights the importance of accurate G6PD deficiency screening in neonates in the Rasht region.
Abstract

Keywords:
Glucose-6-phosphate dehydrogenasefluorescent spot testqualitative testquantitative test

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