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Phenolic compounds isolated from fermented blueberry juice decrease hepatocellular glucose output and enhance muscle
Abir Nachar1,2, Hoda M Eid1,2,3, Melinda Vinqvist-Tymchuk4
1Natural Health Products and Metabolic Diseases Laboratory, Department of Pharmacology and Physiology, Université de Montréal, Station Centre-Ville, P.O. Box 6128, Montréal, Québec, H3C 3J7, Canada.
Fermented blueberry juice (FJ) and its compounds, particularly catechol, show antidiabetic potential by improving glucose homeostasis. This research identifies key active components for standardizing FJ as a functional food.
Area of Science:
- Biochemistry
- Cell Biology
- Nutraceuticals
Background:
- Fermented blueberry juice (FJ), produced with Serratia vaccinii, demonstrates in vivo and in vitro antidiabetic properties.
- Previous research established the antidiabetic activities of FJ.
Purpose of the Study:
- To investigate the impact of FJ on glucose homeostasis in liver and skeletal muscle cells.
- To identify specific fractions and compounds within FJ responsible for these effects.
Main Methods:
- FJ was fractionated using chromatography.
- Hepatic (H4IIE, HepG2) and skeletal muscle (C2C12) cells were treated with FJ, fractions, and isolated compounds.
- Glucose-6-phosphatase (G6Pase) activity, glucose uptake, and glycogen synthase (GS) activity were measured.
Main Results:
- FJ and its phenolic fractions (F2, F3-1, F3-2) inhibited G6Pase, activated GS, and stimulated glucose uptake.
- Catechol, isolated from active fractions, showed significant bioactivity, decreasing G6Pase and increasing GS activity and glucose uptake.
- Specific reductions in G6Pase and increases in GS and glucose uptake were quantified for FJ, fractions, and catechol.
Conclusions:
- This study identifies novel compounds, including catechol, with potential antidiabetic properties.
- The findings support the standardization of FJ as a functional food for managing diabetes.
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