Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

24.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.9K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.9K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

5.1K
5.1K
Experimental RNAi02:15

Experimental RNAi

8.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.1K
RNA Interference01:23

RNA Interference

28.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acceleration of Singlet Oxygen Evolution by Sonopiezoelectric Charge Transfer Over SrTiO<sub>3</sub>-TiO<sub>2</sub> Heterojunction for Selective Oxidation.

Exploration (Beijing, China)·2026
Same author

Correction: CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3.

PLoS pathogens·2026
Same author

Epidemiological investigation of PCV2 and PCV3 in three provinces of Northeast China in 2025.

BMC veterinary research·2026
Same author

Influence of Coupled Activated Recycled Fine Powder on the Performance of Ultra-High-Performance Concrete.

Materials (Basel, Switzerland)·2026
Same author

A Robust Gaussian Process Paradigm for Predictive Modeling on Small Data sets in Environmental Science: A Case Study in Ballasted Flocculation.

Environmental science & technology·2025
Same author

Impact of Age and Sex on Anatomical Variations of the Infra-acetabular Screw Corridor: An Imaging Study.

Clinics in orthopedic surgery·2025

Related Experiment Video

Updated: Mar 6, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K

KSHV microRNAs: Tricks of the Devil.

Jie Qin1, Wan Li1, Shou-Jiang Gao2

  • 1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, P.R. China; Key Laboratory of Pathogen Biology of Jiangsu Province, Nanjing Medical University, Nanjing, P.R. China; Department of Microbiology, Nanjing Medical University, Nanjing 211166, P.R. China.

Trends in Microbiology
|March 6, 2017
PubMed
Summary
This summary is machine-generated.

Kaposi's sarcoma-associated herpesvirus (KSHV) microRNAs (miRNAs) are key drivers in Kaposi's sarcoma (KS) development. These viral miRNAs regulate host genes to promote tumor growth, spread, and immune evasion.

Keywords:
AngiogenesisKSHV microRNATumor disseminationViral latency

More Related Videos

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

9.0K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

36.9K

Related Experiment Videos

Last Updated: Mar 6, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

9.0K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

36.9K

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Kaposi's sarcoma-associated herpesvirus (KSHV) causes Kaposi's sarcoma (KS), a vascular tumor common in immunocompromised individuals.
  • KSHV encodes 12 precursor microRNAs (pre-miRNAs) processed into 25 mature microRNAs (miRNAs).
  • KSHV miRNAs are implicated in maintaining viral latency and promoting KS pathogenesis.

Purpose of the Study:

  • To review the biogenesis of microRNAs (miRNAs).
  • To summarize recent findings on the roles and mechanisms of KSHV-encoded miRNAs in Kaposi's sarcoma (KS) development.
  • To highlight how KSHV miRNAs contribute to tumor progression and immune modulation.

Main Methods:

  • Literature review of studies on KSHV miRNA biogenesis, function, and role in KS.
  • Analysis of mechanisms by which KSHV miRNAs regulate viral and host gene expression.
  • Synthesis of current understanding of KSHV miRNA involvement in angiogenesis and immune evasion.

Main Results:

  • KSHV miRNAs play critical roles in maintaining KSHV latency.
  • These viral miRNAs promote angiogenesis and the dissemination of infected cells.
  • KSHV miRNAs interfere with the host immune system, contributing to KS development.

Conclusions:

  • KSHV-encoded miRNAs are significant contributors to the pathogenesis of Kaposi's sarcoma.
  • Understanding KSHV miRNA mechanisms offers potential therapeutic targets for KS.
  • This review consolidates current knowledge on KSHV miRNAs in KS development.