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High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Enzyme hydrolysis kinetics of micro-grinded maize straws
Jianjun Hu1, Yanyan Jing1, Quanguo Zhang1
1Collaborative Innovation Center of Biomass Energy, Henan Agriculture University, Henan Province, Zhengzhou 450002, China.
Abstract:
This study applied micro-grinding to disintegrate the maize straws and then use the micro-grinded straws of particle sizes particle size 53-61, 80-96 or 150-180μm, for subsequent enzyme hydrolysis tests. The reducing sugar productivity was increased with reducing particle size. A kinetic model considering product inhibition was developed as follows t=aln[S]0[S]0-[P]+b[P], where S, P and t are the substrate, enzyme and hydrolysis time, respectively, and a and b are fitting parameters. The initial substrate concentration is proportional to the total exposed surface area. Additionally, the mechanical grinding can increase the biomass affinity for enzyme attack, suggesting the enhanced local action of shearing on the fiber matrix surfaces. The enhanced hydrolysis efficiency of the micro-grinded straws is welcomed by the subsequent refinery steps.
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