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LATS1 suppresses proliferation and invasion of cervical cancer

Jihong Deng1, Wen Zhang1, Shuangyue Liu1

  • 1Department of Gynecology, Kunming Maternity and Child Care Hospital, Kunming, Yunnan 650000, P.R. China.

Molecular Medicine Reports
|March 6, 2017
PubMed

Insights

Large tumor suppressor kinase 1 (LATS1) is downregulated in cervical cancer, potentially acting as a tumor suppressor. Its restoration inhibits cancer cell growth and invasion by regulating key proteins.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Large tumor suppressor kinase 1 (LATS1) loss is linked to various human cancers.
  • The role of LATS1 in cervical cancer pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the clinical significance and biological functions of LATS1 in human cervical cancer.
  • To determine if LATS1 expression levels correlate with cervical cancer progression.

Main Methods:

  • Immunohistochemistry used to assess LATS1 protein expression in 80 cervical cancer tissues.
  • LATS1 was overexpressed in SiHa cells and knocked down in Caski cells.
  • MTT and Matrigel invasion assays evaluated cell proliferation and invasion.
  • Western blotting assessed the expression of p27, cyclin E, matrix metalloproteinase 9 (MMP9), and yes-associated protein 1 (YAP).

Main Results:

  • LATS1 was downregulated in 45% of cervical cancer cases.
  • LATS1 overexpression suppressed cell proliferation and invasion in SiHa cells.
  • LATS1 overexpression upregulated p27 and YAP phosphorylation, while downregulating cyclin E and MMP9.
  • LATS1 depletion in Caski cells produced opposite effects.

Conclusions:

  • LATS1 is downregulated in human cervical cancer, suggesting a tumor-suppressive role.
  • LATS1 may inhibit cervical cancer cell growth and invasion by modulating the expression of p27, cyclin E, MMP9, and YAP.

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