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Published on: January 5, 2024
Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells
Jing Zhao1, Mei Zhang1, Pengcheng He1
1Department of Hematology, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Abstract:
Oridonin is a diterpenoid compound isolated from the medicinal herb Rabdosia rubescens, and has shown marked antitumor effects against different types of cancer. However, the definitive systematic molecular mechanism underlying the antitumor activity of oridonin in multiple myeloma remains to be elucidated. In the present study, cell viability and cytotoxicity were examined to determine the appropriate concentration for proteomic investigation. In addition, cell apoptosis was evaluated using flow cytometry and transmission electron microscopy. A proteomic investigation using a two‑dimensional electrophoresis system and mass spectrometry was performed to identify and characterize the global proteome of the apoptosis induced by oridonin. Of the proteins identified, seven were involved in the anticancer effects of oridonin. Regulation of the expression and function of target proteins, stathmin, dihydrofolate reductase and pyruvate dehydrogenase E1β, may be potential, therapeutic strategies to effectively treat multiple myeloma. These findings provide novel information on the molecular mechanisms underlying the anticancer properties of oridonin in multiple myeloma.
Insights
Oridonin, a compound from Rabdosia rubescens, shows antitumor effects. This study identified key proteins like stathmin, dihydrofolate reductase, and pyruvate dehydrogenase E1β involved in oridonin-induced apoptosis in multiple myeloma.
Area of Science:
- Pharmacology
- Molecular Biology
- Oncology
Background:
- Oridonin, a diterpenoid from Rabdosia rubescens, exhibits significant antitumor activity.
- The precise molecular mechanisms of oridonin's action in multiple myeloma are not fully understood.
Purpose of the Study:
- To elucidate the molecular mechanisms of oridonin's antitumor activity in multiple myeloma.
- To identify proteins involved in oridonin-induced apoptosis.
Main Methods:
- Cell viability, cytotoxicity, and apoptosis assays (flow cytometry, transmission electron microscopy).
- Proteomic analysis using two-dimensional electrophoresis and mass spectrometry.
Main Results:
- Identified seven proteins involved in oridonin's anticancer effects.
- Highlighted stathmin, dihydrofolate reductase, and pyruvate dehydrogenase E1β as key targets.
Conclusions:
- Oridonin induces apoptosis in multiple myeloma cells through specific protein regulation.
- Targeting stathmin, dihydrofolate reductase, and pyruvate dehydrogenase E1β presents potential therapeutic strategies for multiple myeloma.
