Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular analysis of heritable mouse mutations.

E M Rinchik1

  • 1Biology Division, Oak Ridge National Laboratory, TN 37831.

Environmental Health Perspectives
|October 1, 1987
PubMed
Summary

Mouse germ-line mutations serve as vital toxicological markers. Molecular genetic analysis integrates DNA technology with classical genetics to link DNA defects to organismic effects, advancing mammalian biology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequence interpretation. Functional annotation of mouse genome sequences.

Science (New York, N.Y.)·2001
Same author

Point mutations in the murine fumarylacetoacetate hydrolase gene: Animal models for the human genetic disorder hereditary tyrosinemia type 1.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Effects of ENU dosage on mouse strains.

Mammalian genome : official journal of the International Mammalian Genome Society·2000
Same author

Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations.

Mammalian genome : official journal of the International Mammalian Genome Society·2000
Same author

An integrated deletion and physical map encompassing l71Rl, a chromosome 7 locus required for peri-implantation survival in the mouse.

Genomics·2000
Same author

Molecular characterization of radiation- and chemically induced mutations associated with neuromuscular tremors, runting, juvenile lethality, and sperm defects in jdf2 mice.

Mammalian genome : official journal of the International Mammalian Genome Society·1999

Area of Science:

  • Mammalian genetics
  • Developmental toxicology
  • Molecular biology

Background:

  • Germ-line mutations in mice are established biological markers for reproductive and developmental toxicology.
  • Understanding the molecular basis and phenotypic consequences of mutations remains a key challenge in mammalian biology.

Purpose of the Study:

  • To explore the molecular nature of mutations and their link to phenotypes.
  • To highlight the strategy of molecular genetic analysis for studying heritable mouse mutations.
  • To discuss the integrated use of recombinant DNA technology and classical genetics.

Main Methods:

  • Utilizing recombinant DNA technology for gene identification and expression analysis.
  • Employing classical genetic and cytogenetic analysis to correlate DNA defects with organismic outcomes.
  • Applying approaches like specific-gene cloning, random-clone analysis, and insertional mutagenesis.
  • Focusing on radiation-induced mutations in the dilute-short ear (d-se) region of mouse chromosome 9.

Main Results:

  • Demonstrated the successful integration of genetic analysis and molecular biology.
  • Provided a framework for relating genomic DNA structure to gene expression and function.
  • Detailed studies on radiation-induced mutations at the d-se region.

Conclusions:

  • The integrated approach is crucial for understanding mutation mechanisms and effects.
  • Molecular genetic analysis offers a malleable strategy for studying mammalian mutations.
  • This research advances the correlation of DNA defects with complex phenotypes in mammals.

Related Experiment Videos