Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infertility in Males01:23

Infertility in Males

633
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
633

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Mucosal Type II Interferon Limits SARS-CoV-2 Replication in Humans.

medRxiv : the preprint server for health sciences·2026
Same author

Use of drains for colonic decompression in acute colonic pseudo-obstruction.

iGIE : innovation, investigation and insights·2026
Same author

Vanin-1 deficiency enhances host tolerance to influenza infection by modulating cellular redox status.

PNAS nexus·2026
Same author

Bioactive enhanced adjuvant chemokine oligonucleotide nanoparticles (BEACONs) for mucosal vaccination against genital herpes.

Science immunology·2026
Same author

Analysis Of Salivary Herpesviruses Reveals Associations Between HHV-6 And Long COVID Severity.

medRxiv : the preprint server for health sciences·2026
Same author

Niclosamide inhibits the replication of highly pathogenic avian influenza A (H5Nx) viruses and antiviral-resistant mutants.

Antiviral research·2026

Related Experiment Video

Updated: Jun 17, 2026

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
09:11

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

Published on: August 23, 2016

Zika virus causes testicular atrophy.

Ryuta Uraki1, Jesse Hwang1, Kellie Ann Jurado2

  • 1Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.

Science Advances
|March 7, 2017
PubMed
Summary

Zika virus (ZIKV) can persist in male mice testes, leading to testicular atrophy and reduced testosterone. This study highlights potential long-term reproductive issues and non-vector-borne transmission risks in males.

Keywords:
Ifnar1 KO miceLeydig cellTesticular atrophyTestosteroneZika virusflavivirus

More Related Videos

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult
09:39

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Published on: April 26, 2018

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
10:01

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Published on: October 17, 2018

Related Experiment Videos

Last Updated: Jun 17, 2026

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
09:11

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

Published on: August 23, 2016

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult
09:39

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Published on: April 26, 2018

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
10:01

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Published on: October 17, 2018

Area of Science:

  • Virology
  • Immunology
  • Reproductive Biology

Background:

  • Zika virus (ZIKV) is an emerging flavivirus causing fetal abnormalities.
  • ZIKV is known to persist in human semen post-infection.
  • The impact of ZIKV persistence on male reproductive health requires further investigation.

Purpose of the Study:

  • To investigate the consequences of ZIKV persistence in male reproductive organs.
  • To determine the effects of ZIKV infection on testicular function and testosterone levels.

Main Methods:

  • Infection of Ifnar1 mice with a pathogenic ZIKV strain.
  • Monitoring viral replication in blood and testes.
  • Assessing viral presence in the epididymis and sperm.
  • Measuring testicular size and serum testosterone levels.

Main Results:

  • ZIKV replicated persistently in testes, particularly in Leydig cells, even after blood clearance.
  • High viral loads were detected in the epididymal lumen and epithelial cells.
  • Significant testicular atrophy was observed in infected mice by 21 days post-infection.
  • ZIKV infection led to a reduction in serum testosterone levels.

Conclusions:

  • ZIKV persistence in testes can cause progressive testicular atrophy and reduced testosterone.
  • These findings suggest potential impairment of male fertility following ZIKV infection.
  • The study underscores implications for non-vector-borne transmission and long-term reproductive deficiencies in males.