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Updated: Mar 6, 2026

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IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
Published on: July 7, 2015
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An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins
Kai Cai1, Ying Tan, Chunyan Tan
1Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
Abstract:
The iminodiacetate-Ni2+-hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization of hexahistidine-tagged (His-tagged) proteins using PPEIDA was demonstrated.
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