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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Mar 6, 2026

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Stimulated Emission Depletion Microscopy.

Hans Blom¹, Jerker Widengren²

¹Royal Institute of Technology (KTH) , Dept Applied Physics, SciLifeLab, 17165 Solna, Sweden.

Chemical Reviews

|March 7, 2017

Summary

This summary is machine-generated.

Stimulated emission depletion (STED) imaging offers super-resolution microscopy beyond light diffraction limits. This review covers STED

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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

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Published on: March 24, 2014

Area of Science:

Biological Sciences
Microscopy
Biophysics

Background:

Diffraction-unlimited fluorescence microscopy, particularly Stimulated Emission Depletion (STED) imaging, has rapidly advanced biological sciences.
STED imaging provides superior resolution compared to traditional diffraction-limited optical microscopy and electron microscopy.

Purpose of the Study:

To review the evolution and principles of STED imaging.
To compare STED with other super-resolution techniques and highlight its advantages.
To discuss prerequisites for STED experiments and future advancements.

Main Methods:

Review of STED imaging principles and applications.
Comparative analysis of STED with other microscopy techniques (optical super-resolution, diffraction-limited, electron microscopy).
Discussion of instrumentation, sample preparation, and photophysics for STED.

Main Results:

STED imaging has evolved significantly, offering enhanced resolution for biological samples.
Key factors for successful STED include optimized instrumentation, sample preparation, and understanding photophysics.
STED demonstrates potential in neuroscience, plasma membrane biophysics, and clinical diagnostics.

Conclusions:

STED nanoscopy is a powerful tool for advancing biological research.
Continued development of STED imaging will further expand its applications in life sciences.
STED imaging is poised to become increasingly vital across various scientific and diagnostic fields.