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Immunoaffinity-purified opiate receptor specifically binds the delta-class opiate receptor ligand,
D J Carr1, B DeCosta, A E Jacobson
1University of Alabama at Birmingham, Department of Physiology and Biophysics 35294.
FEBS Letters
|November 30, 1987
Abstract:
Using an antibody generated against the opiate receptor on NG108-15 cells, we recently purified the putative receptor from this hybrid cell line. We herein report that the purified receptor complex specifically binds tritiated cis-(+)-3-methylfentanylisothiocyanate (SUPERFIT), with the predominant binding associated with a 58 kDa polypeptide chain. Consistent with these findings is the in situ labeling of a 58 kDa protein with [3H]SUPERFIT on NG108-15 cells.