Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

18.9K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
18.9K
Hybridization of Atomic Orbitals II03:35

Hybridization of Atomic Orbitals II

49.9K
sp3d and sp3d 2 Hybridization
49.9K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

25.1K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
25.1K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

5.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.8K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.2K
4.2K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

3.2K
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First total synthesis and evaluation of the α-glucosidase inhibitory activity of (±)-methyl dihydromarmesinate, an anti-inflammatory phenylpropanoid from <i>Ficus hirta</i> Vahl., and related benzofurans.

Organic & biomolecular chemistry·2026
Same author

New semisynthetic α-glucosidase inhibitor from a doubly-chemically engineered extract.

Natural products and bioprospecting·2025
Same author

1,3,4-oxadiazoles as inhibitors of the atypical member of the BET family bromodomain factor 3 from <i>Trypanosoma cruzi</i> (<i>Tc</i>BDF3).

Frontiers in microbiology·2024
Same author

Multilevel Dynamic System as Molecular Morning-After Timer.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024
Same author

A simple thin-layer chromatography autography for the detection of peroxidase inhibitors.

Journal of food science and technology·2024
Same author

Effect-Directed Synthesis of PhoP/PhoQ Inhibitors to Regulate <i>Salmonella</i> Virulence.

Journal of agricultural and food chemistry·2022

Related Experiment Video

Updated: Mar 6, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

8.0K

Host Amplification in a Dithioacetal-Based Dynamic Covalent Library.

A Gastón Orrillo1, Andrea M Escalante1, Ricardo L E Furlan1,2

  • 1Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario (UNR-CONICET), Ocampo y Esmeralda, Rosario (2000), Argentina.

Organic Letters
|March 7, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces molecular amplification using dithioacetal dynamic libraries for the first time. A homatropine template enabled the selection and amplification of a specific cyclophane host, showcasing a novel responsive library system.

More Related Videos

Curation of Computational Chemical Libraries Demonstrated with Alpha-Amino Acids
08:21

Curation of Computational Chemical Libraries Demonstrated with Alpha-Amino Acids

Published on: April 13, 2022

3.1K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.7K

Related Experiment Videos

Last Updated: Mar 6, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

8.0K
Curation of Computational Chemical Libraries Demonstrated with Alpha-Amino Acids
08:21

Curation of Computational Chemical Libraries Demonstrated with Alpha-Amino Acids

Published on: April 13, 2022

3.1K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.7K

Area of Science:

  • Supramolecular Chemistry
  • Organic Chemistry
  • Chemical Synthesis

Background:

  • Dynamic combinatorial libraries (DCLs) are powerful tools for discovering molecular hosts.
  • Dithioacetal exchange offers a versatile method for constructing DCLs.
  • Responsive DCLs that can be modulated by external stimuli are highly sought after.

Purpose of the Study:

  • To report the first instance of molecular amplification within a dithioacetal-based dynamic library.
  • To demonstrate the utility of dithioacetal exchange for creating responsive DCLs.
  • To characterize the host-guest interaction of an amplified cyclophane.

Main Methods:

  • Construction of a dithioacetal-based dynamic library.
  • Template-directed selection and amplification using homatropine.
  • Isolation and structural characterization of the amplified cyclophane host.
  • Nuclear magnetic resonance (NMR) spectroscopy and isothermal titration calorimetry (ITC) for binding analysis.

Main Results:

  • Successful molecular amplification of a cyclophane host was achieved using a dithioacetal DCL.
  • Homatropine acted as an effective template, inducing selective amplification.
  • The amplified macrocycle formed a 1:1 complex with the homatropine template.
  • This represents the first reported host-guest system involving a dithioacetal cyclophane.

Conclusions:

  • Dithioacetal exchange is a viable strategy for building responsive dynamic libraries capable of molecular amplification.
  • The developed system demonstrates efficient template-directed synthesis of specific macrocyclic hosts.
  • This work opens new avenues for designing smart molecular recognition systems based on dithioacetal chemistry.