Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.6K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.6K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

3.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.4K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

3.8K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
3.8K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.9K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.9K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.7K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.3K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

VIDEO - Visual Integration of Drosophila Enhancer Organization: A tool for integrating and visualizing chromatin accessibility, in vivo transcription factor binding and motif occurrence in tissue-specific differentially expressed genes.

Genetics·2026
Same author

Hedgehog and Drosophila germ cell migration.

Development (Cambridge, England)·2025
Same author

Video - Visual Integration of Drosophila Enhancer Organization: A tool for integrating and visualizing chromatin accessibility, in vivo transcription factor binding and motif occurrence in tissue-specific differentially expressed genes.

bioRxiv : the preprint server for biology·2025
Same author

CrebA regulation of secretory capacity: genome-wide transcription profiling coupled with in vivo DNA binding studies.

Genetics·2025
Same author

Sulfation affects apical extracellular matrix organization during development of the <i>Drosophila</i> embryonic salivary gland tube.

eLife·2025
Same author

CrebA regulation of secretory capacity: Genome-wide transcription profiling coupled with in vivo DNA binding studies.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Mar 6, 2026

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

1.9K

Uncoupling apical constriction from tissue invagination.

SeYeon Chung1, Sangjoon Kim1, Deborah J Andrew1

  • 1Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, United States.

Elife
|March 7, 2017
PubMed
Summary
This summary is machine-generated.

Apical constriction, crucial for epithelial folding, is regulated by transcription factors and myosin accumulation. Disrupting this process still allows tube formation, highlighting intrinsic salivary gland properties.

Keywords:
D. melanogasterDrosophilaapical constrictioncell biologydevelopmental biologyfogfork headmyosinsalivary glandstem cells

More Related Videos

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models
07:49

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

Published on: March 23, 2021

12.7K
Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

12.4K

Related Experiment Videos

Last Updated: Mar 6, 2026

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

1.9K
Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models
07:49

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

Published on: March 23, 2021

12.7K
Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

12.4K

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Apical constriction is a fundamental cell shape change in epithelial tissues.
  • Spatiotemporal regulation of apical constriction during tissue invagination and its role in tube formation are not fully understood.

Purpose of the Study:

  • To investigate the regulation of apical constriction during Drosophila salivary gland invagination.
  • To understand the contribution of apical constriction to tube formation and identify alternative mechanisms driving invagination.

Main Methods:

  • Utilized Drosophila salivary gland (SG) invagination as a model system.
  • Analyzed the role of the Fork head transcription factor and its regulation of folded gastrulation expression.
  • Investigated the spatiotemporal accumulation of Rho kinase and non-muscle myosin II.
  • Examined the effects of disrupted apical constriction on tissue invagination and tube formation.

Main Results:

  • Fork head transcription factor regulates folded gastrulation expression, which is essential for apicomedial accumulation of Rho kinase and non-muscle myosin II.
  • Spatially coordinated or completely blocked apical constriction did not prevent salivary gland internalization and tube formation, though it altered invagination geometry.
  • A tissue-level myosin cable contributes to salivary gland invagination when apical constriction is disrupted.
  • Fully elongated polarized salivary glands can form outside the embryo.

Conclusions:

  • Salivary gland tube formation and elongation are intrinsic properties of the tissue.
  • While apical constriction is important, alternative mechanisms, such as forces from a myosin cable, can drive invagination.
  • The study elucidates the regulatory network controlling apical constriction and its role within the broader context of epithelial morphogenesis.