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Related Experiment Videos

A single base-pair change creates a Chi recombinational hotspot in bacteriophage lambda.

K U Sprague, D H Faulds, G R Smith

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

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    Chi mutations in phage lambda increase recombination rates. These mutations, specifically in the cII gene, arise from a specific DNA change, likely affecting a key protein in the recombination process.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Chi (X) sites are DNA sequences that stimulate homologous recombination in bacteriophages.
    • The Chi (X) phenotype in phage lambda is associated with increased recombination rates mediated by the Escherichia coli Rec system.
    • Mutations affecting Chi sites can alter recombination efficiency.

    Purpose of the Study:

    • To investigate the nature and location of X4+ mutations responsible for the Chi phenotype in phage lambda.
    • To understand the molecular basis by which X4+ mutations enhance recombination.
    • To identify the specific genetic changes leading to the Chi phenotype.

    Main Methods:

    • Genetic mapping using overlapping deletions to locate X+ mutations within the phage lambda cII gene.

    Related Experiment Videos

  • Physical mapping of deletion endpoints.
  • DNA sequence analysis of the relevant deletion segment to identify specific mutations.

    • Main Results:

    • X4+ mutations were found to increase the rate of recombination promoted by the E. coli Rec system.
    • Two independent X+ mutations within the cII gene were identified.
    • Both mutations resulted from the same A-T to T-A transversion, indicating a specific mutational event.

    Conclusions:

    • The A-T to T-A transversion in the cII gene is responsible for the X4+ Chi phenotype.
    • This specific DNA sequence change likely creates a recognition site for a protein crucial for a rate-limiting step in recombination.
    • The findings provide insight into the molecular mechanisms of recombination regulation in phage lambda.