Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.2K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.4K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatin spatial analysis by METALoci unveils sex-determining 3D regulatory hubs.

Nature structural & molecular biology·2026
Same author

TREM2 expression level is critical for microglial state, metabolic capacity and efficacy of TREM2 agonism.

Nature communications·2026
Same author

Personalized CRISPR knock-in cytokine gene therapy to remodel the tumor microenvironment and enhance CAR T cell therapy in solid tumors.

Nature communications·2025
Same author

BACE1 regulates sleep-wake cycle through both enzymatic and non-enzymatic actions.

EMBO reports·2025
Same author

Prime editing corrects the dilated cardiomyopathy causing RBM20-P633L-mutation in human cardiomyocytes.

Molecular therapy. Nucleic acids·2025
Same author

Neuropathy-associated Tecpr2 mutation knock-in mice reveal endolysosomal loss of function phenotypes in neurons and microglia.

Cell death & disease·2025

Related Experiment Video

Updated: Mar 6, 2026

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.2K

Gene editing in mouse zygotes using the CRISPR/Cas9 system.

Benedikt Wefers1, Sanum Bashir2, Jana Rossius3

  • 1German Center for Neurodegenerative Diseases (DZNE), Feodor-Lynen Str. 17, 81377 Munich, Germany; Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.

Methods (San Diego, Calif.)
|March 7, 2017
PubMed
Summary

CRISPR/Cas9 gene editing enables rapid creation of targeted mouse mutants for research. This guide details designing alleles, preparing reagents, editing zygotes, and genotyping offspring for biomedical applications.

Keywords:
CRISPRCas9Gene editingMouseZygotes

More Related Videos

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

10.5K
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
08:48

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Published on: June 24, 2022

5.7K

Related Experiment Videos

Last Updated: Mar 6, 2026

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.2K
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

10.5K
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
08:48

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Published on: June 24, 2022

5.7K

Area of Science:

  • Biomedical Research
  • Genetics
  • Molecular Biology

Background:

  • Targeted mouse mutants are essential tools in biomedical research.
  • CRISPR/Cas9 technology offers a powerful method for precise gene modification.

Purpose of the Study:

  • To provide a comprehensive guide on generating targeted mouse mutants using CRISPR/Cas9.
  • To detail the process from allele design to genotyping.

Main Methods:

  • CRISPR/Cas9 system for inducing double-strand breaks in mouse zygotes.
  • Design of knockout and knockin alleles.
  • Microinjection or electroporation of zygotes.
  • Genotyping of pups and blastocysts using PCR.

Main Results:

  • Successful generation of targeted mouse mutants through CRISPR/Cas9 gene editing.
  • Demonstration of efficient allele design and zygote manipulation.
  • Validation of PCR-based genotyping methods for accuracy.

Conclusions:

  • CRISPR/Cas9 is a streamlined approach for creating genetically modified mice.
  • The described methods facilitate efficient generation and verification of mouse models.
  • This technology accelerates research in various biomedical fields.