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Updated: Mar 6, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of
Meik Kunz1, Thomas Dandekar2, Muhammad Naseem3,4
1Functional Genomics & Systems Biology Group, Department of Bioinformatics, Biocenter, University of Wuerzburg, 97074, Wuerzburg, Germany.
Abstract:
Cytokinins (CKs) play an important role in plant growth and development. Also, several studies highlight the modulatory implications of CKs for plant-pathogen interaction. However, the underlying mechanisms of CK mediating immune networks in plants are still not fully understood. A detailed analysis of high-throughput transcriptome (RNA-Seq and microarrays) datasets under modulated conditions of plant CKs and its mergence with cellular interactome (large-scale protein-protein interaction data) has the potential to unlock the contribution of CKs to plant defense. Here, we specifically describe a detailed systems biology methodology pertinent to the acquisition and analysis of various omics datasets that delineate the role of plant CKs in impacting immune pathways in Arabidopsis.
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