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Quality control of Hypericum perforatum L. analytical challenges and recent progress
Anastasia Agapouda1, Anthony Booker1,2, Tivadar Kiss3,4
1Research Cluster "Biodiversity and Medicines" , Research Group "Pharmacognosy and Phytotherapy" , UCL School of Pharmacy , University of London, London, UK.
The Journal of Pharmacy and Pharmacology
|March 8, 2017
Summary
This review covers analytical methods for Hypericum perforatum (St. John's wort) extract quality control. HPLC-DAD is versatile for quantitative analysis, while newer methods like LC-MS and NMR metabolomics offer deeper insights.
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Pharmacognosy
Background:
- Hypericum perforatum (St. John's wort) possesses diverse biologically active compounds.
- Comprehensive analysis requires various chromatographic and detection techniques.
- Key analyzed metabolites include naphthodianthrones, phloroglucinols, and flavonoids.
Purpose of the Study:
- To review qualitative and quantitative analytical methods for Hypericum perforatum extract quality control.
- To discuss routine analytical tools and modern approaches.
- To highlight methods for analyzing major and minor compounds.
Main Methods:
- Thin-Layer Chromatography (TLC)
- High-Performance Thin-Layer Chromatography (HPTLC)
- High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- DNA barcoding
- Near-Infrared Spectroscopy (NIRS)
- Nuclear Magnetic Resonance (NMR) metabolomics
Main Results:
- TLC and HPTLC are fundamental for routine analysis.
- HPLC-DAD is the predominant method for quantitative analysis due to its flexibility.
- LC-MS is increasingly utilized in pharmacokinetic studies for its high sensitivity.
Conclusions:
- Routine quality control often focuses on major compounds.
- Detailed characterization necessitates the analysis of minor compounds.
- Emerging techniques like DNA barcoding, NIRS, and NMR metabolomics provide advanced characterization of secondary metabolite profiles.
