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Profiling analysis of long non-coding RNAs in early postnatal mouse hearts
Xiongshan Sun1,2, Qi Han1,2, Hongqin Luo1,2
1Institute of Materia Medica, College of Pharmacy, Third Military Medical University, Chongqing, China.
Scientific Reports
|March 8, 2017
Summary
This study identifies long non-coding RNAs (lncRNAs) involved in the critical transition of cardiomyocyte growth in early postnatal mouse hearts. One novel lncRNA, ENSMUST00000117266, regulates cardiomyocyte proliferation during this crucial developmental stage.
Area of Science:
- Cardiovascular Biology
- Molecular Genetics
- Developmental Biology
Background:
- Mammalian cardiomyocyte growth transitions from hyperplasia to hypertrophy postnatally.
- This transition is vital for establishing normal heart function.
- The role of long non-coding RNAs (lncRNAs) in this process is largely unexplored.
Purpose of the Study:
- To investigate the role of lncRNAs in the postnatal hyperplastic-to-hypertrophic growth transition of cardiomyocytes.
- To identify specific lncRNAs involved in regulating cardiomyocyte proliferation during this critical window.
Main Methods:
- Microarray analysis of lncRNA expression profiles in mouse hearts at postnatal days 1, 7, and 28.
- Differential expression analysis to identify significant lncRNAs.
- Knockdown of a novel cardiac-enriched lncRNA (ENSMUST00000117266) and assessment of its impact on cardiomyocyte cell cycle phases.
Main Results:
- Identified 1,146 differentially expressed lncRNAs between postnatal days 1, 7, and 28.
- Neighboring genes of differentially expressed lncRNAs are primarily involved in DNA replication.
- Knockdown of ENSMUST00000117266 increased G0/G1 phase and decreased G2/M phase in neonatal cardiomyocytes, indicating a role in proliferation regulation.
Conclusions:
- A significant number of lncRNAs are expressed in the early postnatal mouse heart.
- ENSMUST00000117266 plays a role in regulating cardiomyocyte proliferative activity.
- These identified lncRNAs may be crucial for the cardiac hyperplastic-to-hypertrophic growth transition.
