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In vivo microsampling to capture the elusive exposome
Vincent Bessonneau1, Jennifer Ings2, Mark McMaster2
1Department of Chemistry, University of Waterloo, ON, Canada.
Scientific Reports
|March 8, 2017
Summary
In vivo solid-phase microextraction (SPME) sampling captures more small molecules from fish tissue than ex vivo methods. This technique improves exposome analysis reliability and enables longitudinal studies.
Area of Science:
- Environmental chemistry
- Analytical chemistry
- Biomonitoring
Background:
- Small molecule loss during sample handling impacts metabolomics data interpretation.
- Exposome research requires robust methods for capturing diverse molecules.
Purpose of the Study:
- To compare in vivo and ex vivo SPME sampling for fish tissue exposome analysis.
- To evaluate the effectiveness of in vivo SPME for stabilizing reactive small molecules.
Main Methods:
- In vivo and ex vivo solid-phase microextraction (SPME) sampling of fish tissue.
- Non-targeted liquid chromatography-high-resolution tandem mass spectrometry (LC-MS/MS).
- Molecular networking analysis for exposome characterization.
Main Results:
- In vivo SPME demonstrated superior extraction and stabilization of reactive molecules like phospholipids.
- 494 MS/MS spectra comparisons confirmed differences between in vivo and ex vivo methods.
- Reduced sample handling in vivo minimizes analyte degradation.
Conclusions:
- In vivo SPME sampling enhances the accuracy and reliability of fish tissue exposome assessment.
- This method facilitates longitudinal biomonitoring and improves exposome-wide association studies.
