Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Replisome03:01

The Replisome

39.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
39.1K
The Replisome03:01

The Replisome

10.8K
10.8K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.4K
2.4K
The DNA Replication Fork01:02

The DNA Replication Fork

42.0K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
42.0K
The DNA Replication Fork01:02

The DNA Replication Fork

19.0K
19.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The E. coli DnaX clamp loader sharply bends DNA to load β-clamp at nicks and small gaps.

Molecular cell·2026
Same author

The <i>E. coli</i> DnaX clamp loader sharply bends DNA to load β-clamp at nicks and small gaps.

bioRxiv : the preprint server for biology·2026
Same author

A non-catalytic role for RFC in PCNA-mediated processive DNA synthesis.

Cell·2026
Same author

Author Correction: Structures of the human leading strand Polε-PCNA holoenzyme.

Nature communications·2024
Same author

Structures of the human leading strand Polε-PCNA holoenzyme.

Nature communications·2024
Same author

Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis.

Science (New York, N.Y.)·2024
Same journal

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same journal

Sub-second fluctuations between top-down and bottom-up modes distinguish diverse human brain states.

Current biology : CB·2026
Same journal

Queen bees offload pesticide burden to eggs when social buffering is overwhelmed.

Current biology : CB·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
See all related articles

Related Experiment Video

Updated: Mar 6, 2026

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.6K

DNA Replication: How Does a Sliding Clamp Slide?

Nina Y Yao1, Mike O'Donnell2

  • 1The Rockefeller University, 1230 York Avenue, New York City, NY 10065, USA.

Current Biology : CB
|March 8, 2017
PubMed
Summary
This summary is machine-generated.

Human PCNA sliding clamps, essential for DNA replication, were structurally and dynamically analyzed. This research reveals the precise mechanism by which these vital protein rings move along DNA strands.

More Related Videos

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.7K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Related Experiment Videos

Last Updated: Mar 6, 2026

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.6K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.7K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • DNA sliding clamps are crucial protein structures that encircle DNA.
  • These clamps tether enzymes, facilitating DNA replication and repair.
  • The mechanism of sliding clamp movement on DNA has been poorly understood.

Purpose of the Study:

  • To elucidate the molecular mechanism of how human PCNA (Proliferating Cell Nuclear Antigen) slides on DNA.
  • To provide a structural and dynamic basis for clamp-DNA interaction.

Main Methods:

  • X-ray crystallography to determine the high-resolution structure of PCNA bound to DNA.
  • Molecular dynamics simulations to observe the dynamic behavior of the clamp-DNA complex.
  • Nuclear Magnetic Resonance (NMR) spectroscopy to further investigate protein-DNA interactions.

Main Results:

  • A detailed crystal structure revealed the atomic interactions between PCNA and DNA.
  • Molecular dynamics simulations showed the dynamic process of clamp translocation.
  • NMR studies confirmed key interactions and conformational changes.

Conclusions:

  • The study provides a comprehensive mechanistic understanding of PCNA sliding on DNA.
  • This reveals how PCNA tethers enzymes during DNA processes.
  • The findings offer insights into the regulation of DNA replication and repair.