Jove
Visualize
Contact Us

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic potential of ultrasound for spinal cord injury.

Neural regeneration research·2025
Same author

PROTOCOL: Association of Antenatal Cytokine Concentrations With Neurodevelopmental Disorders of the Offspring: A Scoping Review.

Campbell systematic reviews·2025
Same author

Lab on a bead with oscillatory centrifugal microfluidics for fast and complete mixing enables fast and accurate biomedical assays.

Scientific reports·2024
Same author

Portable System for In-Clinic Differentiation of Skin Cancers from Benign Skin Lesions and Inflammatory Dermatoses.

JID innovations : skin science from molecules to population health·2024
Same author

Utilizing Deep Learning Algorithms for Signal Processing in Electrochemical Biosensors: From Data Augmentation to Detection and Quantification of Chemicals of Interest.

Bioengineering (Basel, Switzerland)·2023
Same author

UV Laser Stimulation of Ca<sup>2+</sup> Transients in Aggressive Glioblastoma Brain Cancer Cells<sup></sup>.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2023
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 6, 2026

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
07:19

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Published on: March 7, 2014

13.9K

Investigating parylene-HT as a substrate for human cell patterning.

Brad J Raos, E Scott Graham, Alan F Murray

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |March 9, 2017
    PubMed
    Summary

    Parylene-HT on SiO2 substrates effectively patterns human astrocytes, showing cells prefer Parylene-HT over SiO2. This offers a new, UV and thermally resistant platform for cell patterning applications.

    More Related Videos

    A Versatile Method of Patterning Proteins and Cells
    09:57

    A Versatile Method of Patterning Proteins and Cells

    Published on: February 26, 2017

    9.8K
    Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
    09:30

    Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

    Published on: June 2, 2022

    3.0K

    Related Experiment Videos

    Last Updated: Mar 6, 2026

    Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
    07:19

    Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

    Published on: March 7, 2014

    13.9K
    A Versatile Method of Patterning Proteins and Cells
    09:57

    A Versatile Method of Patterning Proteins and Cells

    Published on: February 26, 2017

    9.8K
    Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
    09:30

    Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

    Published on: June 2, 2022

    3.0K

    Area of Science:

    • Biomaterials Science
    • Cell Biology
    • Surface Chemistry

    Background:

    • Cell patterning is crucial for understanding cell behavior and developing tissue engineering strategies.
    • Standard cell culture substrates often lack the specific properties required for precise cell adhesion control.
    • Parylene-C is a known biocompatible coating, but its limitations in UV and thermal resistance hinder certain applications.

    Purpose of the Study:

    • To investigate the potential of Parylene-HT as a novel human cell patterning platform.
    • To evaluate the cell adhesion and morphology of human astrocytes on Parylene-HT and SiO2 substrates.
    • To determine the effectiveness of Parylene-HT in achieving controlled cell distribution.

    Main Methods:

    • Human astrocytes (hNT) derived from the human NTera2.D1 cell line were cultured on SiO2 substrates coated with Parylene-HT.
    • Cell adhesion, morphology, and distribution were analyzed using microscopy.
    • The differential adhesion of cells to Parylene-HT versus SiO2 was quantified.

    Main Results:

    • Human astrocytes exhibited selective adhesion, being attracted to Parylene-HT and repelled by SiO2.
    • Astrocytes on Parylene-HT adopted morphologies comparable to those on standard tissue culture polystyrene.
    • A significant cell patterning ratio of 8:1 (Parylene-HT:SiO2) was achieved, demonstrating effective patterning.

    Conclusions:

    • Parylene-HT on SiO2 serves as a viable and effective platform for human cell patterning.
    • The material properties of Parylene-HT, including its UV and thermal resistance, make it a superior alternative to Parylene-C for cell patterning.
    • This platform holds promise for advanced applications in neuroscience and regenerative medicine requiring precise cell placement.