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Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Immunoprecipitation01:20

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Related Experiment Video

Updated: Mar 6, 2026

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
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Fine Tuning Antibody Conjugation Methods using SNAP-tag Technology.

Karinna Chouman1, Mira Woitok2, Radoslav Mladenov1

  • 1Department of Pharmaceutical Product Development, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074, Aachen. Germany.

Anti-Cancer Agents in Medicinal Chemistry
|March 9, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a new method for creating uniform cancer theranostic agents. This approach enables precise imaging and phototherapy of cancer cells, improving personalized medicine.

Keywords:
EGFRIRDye700SNAP-tag technologyTheranosticsantibody drug conjugatephotodynamic therapysite-directed labeling method

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Area of Science:

  • Bioconjugation Chemistry
  • Molecular Imaging
  • Cancer Therapeutics

Background:

  • Targeted imaging and therapy (theranostics) offers improved cancer diagnosis, prognosis, and management.
  • Antibody-drug conjugates are key to personalized cancer medicine, but their development requires optimization of conjugation methods and drug-to-antibody ratios.
  • Homogeneous immunotheranostic reagents are crucial for effective and predictable cancer treatment.

Purpose of the Study:

  • To develop a versatile technology platform for generating homogeneous immunotheranostic reagents.
  • To address key challenges in creating antibody-drug conjugates with controlled properties.
  • To establish a robust method for site-directed labeling in immunoconjugate development.

Main Methods:

  • Conjugation of the theranostic reagent IRDye700dx to a recombinant antibody fusion protein.
  • Utilizing a self-labeling protein (SNAP-tag) for a unique and controlled reaction site.
  • Developing a site-directed labeling strategy for homogeneous immunoconjugate generation.

Main Results:

  • The developed conjugate demonstrated effective imaging of cancer cells expressing the epidermal growth factor receptor.
  • The immunoconjugate exhibited potent phototherapeutic activity against targeted cancer cells.
  • Successful generation of a homogeneous immunoconjugate with defined pharmacological properties.

Conclusions:

  • A simple, rapid, and robust site-directed labeling method for immunoconjugates has been established.
  • This method facilitates the generation of homogeneous immunoconjugates with predictable therapeutic and imaging capabilities.
  • The developed platform supports the advancement of personalized cancer theranostics.