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Functional Human Beige Adipocytes From Induced Pluripotent Stem Cells.

Anne-Claire Guénantin1,2, Nolwenn Briand1, Emilie Capel3

  • 1Sorbonne Universités, Université Pierre et Marie Curie, INSERM UMR_S938, Centre de Recherche Saint-Antoine, Institute of Cardiometabolism and Nutrition, Paris, France anne-claire.guenantin@inserm.fr nolwenn.briand@inserm.fr.

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Summary

Scientists developed an efficient method to create human beige adipocytes from stem cells. These cells are functional and can be used for disease modeling and drug discovery for obesity and diabetes.

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Area of Science:

  • Cell Biology
  • Stem Cell Research
  • Metabolic Diseases

Background:

  • Thermogenic beige adipocytes are a key target for treating obesity and diabetes.
  • Developing human models for beige adipocyte differentiation is crucial for therapeutic strategies.

Purpose of the Study:

  • To establish a straightforward and efficient protocol for generating functional human beige adipocytes from human induced pluripotent stem cells (hiPSCs).
  • To provide a scalable model for studying beige adipocyte development and for therapeutic screening.

Main Methods:

  • Utilized a protocol to differentiate hiPSCs into beige adipocytes without overexpressing exogenous genes.
  • The differentiation process involved successive mesodermal and adipogenic progenitor stages.
  • Characterized the functional properties of hiPSC-derived beige adipocytes, including gene expression, mitochondrial content, and oxygen consumption.

Main Results:

  • Generated functional human beige adipocytes from hiPSCs that are insulin sensitive.
  • These cells exhibited beige-specific markers and thermogenic gene upregulation.
  • Engrafted hiPSC-derived adipocytes formed vascularized adipose tissue in mice, showing responsive glucose uptake.

Conclusions:

  • The developed protocol efficiently generates functional human beige adipocytes from hiPSCs.
  • This model offers a valuable tool for disease modeling and therapeutic screening for metabolic disorders.
  • The hiPSC-derived beige adipocytes demonstrate key functional characteristics and in vivo potential.