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Related Experiment Video

Updated: Mar 6, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

Protein profiles of hatchery egg shell membrane.

N C Rath¹, R Liyanage², S K Makkar³

¹USDA/Agricultural Research Service, Poultry Production and Product Safety Research Unit, Fayetteville, AR 72701 USA.

Proteome Science

|March 9, 2017

Summary

Hatchery eggshell membranes (HESM) contain diverse bioactive proteins and microbial proteins. This protein-rich poultry waste shows potential for improving post-hatch immunity in chickens.

Keywords:

Hatchery egg shell membrane Mass spectrometry Proteins

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Last Updated: Mar 6, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

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Published on: January 27, 2021

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Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Area of Science:

Poultry Science
Biochemistry
Proteomics

Background:

Eggshells are a significant poultry hatchery waste product.
Eggshell membranes (ESM) contain proteins from egg whites, embryos, and contaminants.
Hatchery eggshell membranes (HESM) show potential as feed supplements for poultry health.

Purpose of the Study:

To identify the protein profiles of hatchery eggshell membranes (HESM).
To understand the bioactive components responsible for HESM's health benefits.

Main Methods:

Extraction of HESM using acidified methanol and guanidine hydrochloride buffer.
Protein analysis via MALDI-TOF-MS and LC-MS-MS after reduction, alkylation, and trypsin digestion.

Main Results:

Identified abundant egg white proteins (ovalbumin, ovotransferrin, lysozyme).
Detected proteins involved in cytoskeletal, cell signaling, and metabolic functions.
Found blood-derived proteins and diverse bacterial proteins (commensal and zoonotic).

Conclusions:

HESM possess a variety of bioactive proteins, including signaling and enzymatic types.
Diverse microbial proteins in HESM contribute to its biological potential.
HESM are suitable for nutritional and biological applications to enhance poultry immunity.