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Updated: Mar 6, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Multifrequency-swept optical coherence microscopy for highspeed full-field tomographic vibrometry in biological
Samuel Choi1, Keita Sato2, Takeru Ota3
1Niigata University, Department of Electrical and Electronics Engineering, 8050 Ikarashi-2, Niigata 950-2181, Japan; AMED-CTRST, AMED, Japan.
Abstract:
Because conventional laser Doppler vibrometry or Doppler optical coherence tomography require mechanical scanning probes that cannot simultaneously measure the wide-range dynamics of bio-tissues, a multifrequency-swept optical coherence microscopy with wide-field heterodyne detection technique was developed. A 1024 × 1024 × 2000 voxel volume was acquired with an axial resolution of ~1.8 μm and an acquisition speed of 2 s. Vibration measurements at 10 kHz were performed over a wide field of view. Wide-field tomographic vibration measurements of a mouse tympanic membrane are demonstrated to illustrate the applicability of this method to live animals.
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