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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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Atomic Force Microscopy01:08

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Related Experiment Video

Updated: Mar 6, 2026

Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model
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Core/shell nanofiber characterization by Raman scanning microscopy.

Lauren Sfakis1, Anna Sharikova2, David Tuschel3

  • 1SUNY Polytechnic Institute, Nanobioscience Constellation, Albany NY, USA.

Biomedical Optics Express
|March 9, 2017
PubMed
Summary

Core/shell nanofibers offer versatile tissue engineering scaffolds. Confocal Raman microscopy provides a non-invasive method to characterize these Poly (glycerol-sebacate)/Poly (lactic-co-glycolic) nanofibers at the nanoscale.

Keywords:
(160.4236) Nanomaterials(160.5470) Polymers(180.5655) Raman microscopy(300.6450) Spectroscopy, Raman

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background:

  • Core/shell nanofibers are increasingly utilized in tissue engineering due to their tunable mechanical and degradation properties.
  • These nanofibers mimic native tissue extracellular matrix, promoting cellular attachment and integration.
  • Characterizing and quantifying these complex nanofiber structures post-fabrication presents a significant challenge.

Purpose of the Study:

  • To develop and validate a non-invasive method for nanoscale composition characterization and quantification of core/shell nanofibers.
  • To assess the utility of Confocal Raman microscopy for analyzing Poly (glycerol-sebacate)/Poly (lactic-co-glycolic) (PGS/PLGA) nanofibers.
  • To ensure the quality of nanofiber scaffolds for tissue engineering applications.

Main Methods:

  • Development of a non-invasive Confocal Raman microscopy technique.
  • Application of the technique for nanoscale characterization of Poly (glycerol-sebacate)/Poly (lactic-co-glycolic) (PGS/PLGA) core/shell nanofibers.
  • Quantification of fiber composition and structure.

Main Results:

  • Successful implementation of Confocal Raman microscopy for non-invasive characterization.
  • Accurate nanoscale quantification of PGS/PLGA core/shell nanofiber composition.
  • Demonstration of the method's efficiency in analyzing scaffold quality.

Conclusions:

  • Confocal Raman microscopy offers an effective, non-invasive approach for characterizing core/shell nanofibers.
  • This method enables rapid quality assessment of biomaterial scaffolds for tissue engineering.
  • The developed technique supports the advancement of biodegradable and biocompatible nanofiber applications.