A protoplast transformation system for Listeria sp
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M F Vicente1, F Baquero, J C Perez-Diaz
1Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain.
Plasmid
|July 1, 1987
Abstract:
We describe protoplast transformation of members of the genus Listeria with plasmid DNA. Shuttle vectors able to replicate in both Escherichia coli and Listeria have been constructed by fusing the E. coli plasmid pUC8 with the Bacillus subtilis plasmids pBD9 and pBD10.