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DNA-only Transposons

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...

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Related Experiment Video

Updated: Mar 6, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Complex multi-enhancer contacts captured by genome architecture mapping.

Robert A Beagrie^1,2,3, Antonio Scialdone⁴, Markus Schueler¹

¹Epigenetic Regulation and Chromatin Architecture Group, Berlin Institute for Medical Systems Biology, Max-Delbrück Centre for Molecular Medicine, Robert-Rössle Straße, Berlin-Buch 13125, Germany.

|March 9, 2017

Summary

This summary is machine-generated.

Genome architecture mapping (GAM) reveals how genes and regulatory elements interact in 3D space within the nucleus. This new method provides unprecedented insight into genome organization and its role in gene expression.

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Last Updated: Mar 6, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

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Published on: June 28, 2018

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

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Published on: November 3, 2023

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Area of Science:

Genomics
Molecular Biology
Cell Biology

Background:

Gene regulation relies on the 3D organization of the genome within the nucleus.
Disruptions in genome organization and gene-regulatory element interactions are linked to disease.
Current technologies have limitations in fully capturing genome architecture.

Purpose of the Study:

To introduce a novel genome-wide method, Genome Architecture Mapping (GAM), for measuring 3D chromatin topology.
To investigate interactions between genes and regulatory elements across large genomic distances.
To explore higher-order chromatin contacts and their role in genome organization.

Main Methods:

Genome Architecture Mapping (GAM) involves sequencing DNA from thin nuclear sections.
Application of GAM to mouse embryonic stem cells.
Utilizing the SLICE (statistical inference of co-segregation) mathematical model to analyze chromatin contacts.

Main Results:

GAM identified specific interactions between active genes and enhancers across large genomic distances.
The study revealed an abundance of three-way chromatin contacts, particularly involving highly transcribed regions and super-enhancers.
GAM provided new insights into genome architecture previously unattainable with existing technologies.

Conclusions:

GAM is a powerful new method for studying 3D genome organization.
Gene-expression-specific contacts play a significant role in organizing the mammalian nucleus.
Understanding genome architecture is crucial for comprehending gene regulation and disease mechanisms.