Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preliminary effects of intermittent theta burst stimulation combined with virtual reality-based cycling training on upper extremity function in children with cerebral palsy: a double-blind randomized crossover trial.

BMC neurology·2026
Same author

Motor differences in jumping among children with and without autism spectrum disorder.

Clinical biomechanics (Bristol, Avon)·2026
Same author

ENT1 inhibitor J4 restores cognitive function and white-matter integrity in a mouse model of tuberous sclerosis complex.

Journal of biomedical science·2026
Same author

Enhancing cancer vaccine efficacy via electrostatic engineering of an FcγR-targeted protein.

Frontiers in immunology·2026
Same author

Comparison of Treatment Outcomes From 6 Weeks of Home-Based Kinesio Taping and Transcutaneous Electrical Nerve Stimulation Combined With Self-Applied Myofascial Stretching in Adults With Carpal Tunnel Syndrome.

Medical science monitor : international medical journal of experimental and clinical research·2026
Same author

Hyperspectral reconstruction of white light endoscopy images for enhanced segmentation of early esophageal lesions in patients.

iScience·2026

Related Experiment Video

Updated: Mar 6, 2026

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

7.2K

Dengue virus infection increases microglial cell migration.

Ming-Kai Jhan1,2, Tsung-Ting Tsai2, Chia-Ling Chen3

  • 1Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, 110, Taiwan.

Scientific Reports
|March 10, 2017
PubMed
Summary

Dengue virus (DENV) infects and increases microglial cell migration by entering cells via clathrin-mediated endocytosis and activating toll-like receptor 3 (TLR3) signaling. This study clarifies DENV

More Related Videos

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

35.3K
Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
06:00

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

2.2K

Related Experiment Videos

Last Updated: Mar 6, 2026

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

7.2K
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

35.3K
Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
06:00

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

2.2K

Area of Science:

  • Neuroimmunology
  • Virology
  • Cell Biology

Background:

  • Activated microglia are observed in dengue virus (DENV)-infected brains, but DENV's specific impact on microglial function is not fully understood.
  • Microglia play crucial roles in central nervous system immunity and inflammation.

Purpose of the Study:

  • To investigate the effects of DENV infection on microglial cell behavior, specifically focusing on cell morphology, motility, and migration.
  • To elucidate the mechanisms underlying DENV-induced microglial migration, including the roles of viral entry pathways and immune receptor signaling.

Main Methods:

  • Utilized the murine microglial cell line BV2 for in vitro infection studies with DENV.
  • Assessed viral replication, protein expression, cell morphology (multipolar phenotype), cell motility, and migration.
  • Investigated the involvement of actin filaments, clathrin-mediated endocytosis, and toll-like receptor 3 (TLR3) signaling pathways.

Main Results:

  • DENV successfully infected BV2 cells, leading to viral entry, RNA replication, protein expression, and virus release.
  • DENV infection induced a multipolar phenotype and significantly increased microglial cell motility and migration.
  • DENV-induced migration was dependent on clathrin-mediated endocytosis for viral entry and involved TLR3 signaling activation.

Conclusions:

  • Dengue virus infection directly impacts microglial cells, promoting migration through a mechanism involving clathrin-mediated endocytosis and TLR3 pathway activation.
  • These findings reveal a novel aspect of neuroinflammation during DENV infection, highlighting microglia's altered migratory response.