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Updated: Mar 6, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Brucella suis carbonic anhydrases and their inhibitors: Towards alternative antibiotics?
Stephan Köhler1, Safia Ouahrani-Bettache1, Jean-Yves Winum2
1a Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, Université de Montpellier, CNRS , Montpellier , France.
Abstract:
Carbonic anhydrases have started to emerge as new potential antibacterial targets for several pathogens. Two β-carbonic anhydrases, denominated bsCA I and bsCA II, have been isolated and characterized from the bacterial pathogen Brucella suis, the causative agent of brucellosis or Malta fever. These enzymes have been investigated in detail and a wide range of classical aromatic and heteroaromatic sulfonamides as well as carbohydrate-based compounds have been found to inhibit selectively and efficiently Brucella suis carbonic anhydrases. Inhibition of these metalloenzymes constitutes a novel approach for the potential development of new anti-Brucella agents. This review aims at discussing the recent literature on this topic.
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