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Updated: Mar 6, 2026

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

Published on: July 18, 2011

Assays for Mitophagy in Yeast.

Akinori Eiyama¹, Koji Okamoto²

¹Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, 565-0871, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|March 10, 2017

Summary

Mitophagy, the selective removal of damaged mitochondria, is vital for cellular health. This study validates experimental methods in yeast to analyze mitophagy, offering insights into conserved degradation mechanisms.

Keywords:

Mitochondria degradation Mitophagosome Mitophagy Respiration Yeast

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A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

Published on: July 18, 2011

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

Published on: May 19, 2023

Area of Science:

Cell Biology
Molecular Biology
Autophagy Research

Background:

Mitochondrial quality control is essential for cellular integrity and energy homeostasis.
Mitophagy, a selective form of autophagy, degrades damaged or surplus mitochondria.
This process is conserved across eukaryotes, making yeast a valuable model organism.

Purpose of the Study:

To introduce and validate experimental approaches for studying mitophagy in Saccharomyces cerevisiae.
To provide methods for visualizing and quantifying mitochondrial degradation via mitophagy.

Main Methods:

Fluorescence microscopy to observe mitochondria transport to the vacuole and autophagosome formation.
Western blotting to quantify the rate of mitochondrial degradation.

Main Results:

Experimental protocols were established to validate mitophagy in yeast.
Fluorescence microscopy successfully visualized mitophagy-related events.
Western blotting provided a quantitative measure of mitochondrial degradation.

Conclusions:

The presented methods enable robust validation of mitophagy in yeast.
Yeast serves as a powerful model for dissecting conserved mitophagy pathways.
These techniques can advance our understanding of mitochondrial quality control mechanisms.