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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Mar 6, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

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Development of fluorescent methods for DNA methyltransferase assay.

Yueying Li, Xiaoran Zou, Fei Ma

    Methods and Applications in Fluorescence
    |March 10, 2017
    PubMed
    Summary

    Accurate detection of DNA methyltransferase (MTase) activity is vital for disease diagnosis. This review highlights recent fluorescent methods, offering simpler and more sensitive assays for DNA MTase activity, crucial for biomedical research and clinical applications.

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    Antibody-Free Assay for RNA Methyltransferase Activity Analysis
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    Antibody-Free Assay for RNA Methyltransferase Activity Analysis

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Analytical Chemistry

    Background:

    • DNA methylation, regulated by DNA methyltransferase (MTase), is essential for gene transcription, cell growth, and proliferation.
    • Aberrant DNA MTase activity is linked to human diseases, particularly cancers, necessitating precise activity detection.
    • Conventional DNA MTase assays are often laborious and time-consuming, limiting their widespread clinical use.

    Purpose of the Study:

    • To review recent advancements in fluorescent-based methods for assaying DNA methyltransferase (MTase) activity.
    • To highlight the advantages of fluorescent assays, including simplicity and high sensitivity, over traditional methods.
    • To discuss emerging amplification-free and amplification-assisted fluorescent assays for DNA MTase detection.

    Main Methods:

    • Summarizing recent literature on fluorescent probes and techniques for DNA MTase activity measurement.
    • Categorizing methods into amplification-free and amplification-assisted fluorescent assays.
    • Analyzing the principles, advantages, and limitations of various fluorescent detection strategies.

    Main Results:

    • Fluorescent methods offer a sensitive, simple, and rapid alternative to conventional DNA MTase assays.
    • Both amplification-free and amplification-assisted fluorescent assays have shown significant progress.
    • These methods enable more efficient monitoring of DNA MTase activity in various biological contexts.

    Conclusions:

    • Fluorescent assays represent a significant breakthrough in the sensitive and efficient detection of DNA MTase activity.
    • Continued development in this area promises improved tools for biomedical research, diagnostics, and therapeutic strategies.
    • Addressing current challenges will further enhance the utility and application of these advanced assays.