Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subunit interactions in the first component of complement, C1.

S Lakatos1

  • 1Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest.

Biochemical and Biophysical Research Communications
|December 16, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Platelet adhesion and fibrinogen deposition in murine microvessels upon inhalation of nanosized carbon particles.

Journal of thrombosis and haemostasis : JTH·2010
Same author

Azurophil granules are heterogeneous with respect to mobilization induced by different concentrations of fMLP.

Haematologia·2002
Same author

fMLP-induced respiratory burst and the intracellular Ca2+ signal are not interrelated in neutrophils.

Haematologia·2001
Same author

A common perceptual space for harmonic and percussive timbres.

Perception & psychophysics·2001
Same author

Drought stress, peroxidase activity and formaldehyde metabolism in bean plants.

Acta biologica Hungarica·1999
Same author

Haptic form perception: relative salience of local and global features.

Perception & psychophysics·1999
Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026
Same journal

COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma.

Biochemical and biophysical research communications·2026
Same journal

Tumor budding in colorectal cancer: partial EMT, microenvironmental remodeling, and metastatic competence.

Biochemical and biophysical research communications·2026
Same journal

Exploring the therapeutic versatility and multitarget pharmacological potential of acyl hydrazone-hydrazide scaffolds.

Biochemical and biophysical research communications·2026
Same journal

The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

Biochemical and biophysical research communications·2026
Same journal

MiR-425-5p modulation of CREB1 affects inflammatory response and motor recovery after spinal cord injury.

Biochemical and biophysical research communications·2026
See all related articles

The complement system

Area of Science:

  • Biochemistry
  • Immunology
  • Molecular Biology

Background:

  • The complement system is a crucial part of the innate immune system.
  • Component 1 (C1) is the first component of the classical complement pathway.
  • C1 is a large complex composed of C1q, C1r, and C1s subunits.

Purpose of the Study:

  • To investigate the binding interactions between C1q and other subunits of C1.
  • To determine the affinity and cooperativity of these interactions.
  • To understand how activation of C1 affects these interactions.

Main Methods:

  • Sucrose gradient ultracentrifugation was used to analyze subunit interactions.
  • Radioiodine-labeled C1q was sedimented through solutions containing different C1 subunits.

Related Experiment Videos

  • Computer modeling was employed to analyze binding mechanisms.

    • Main Results:

    • C1q binds strongly to the C1r2 moiety of the C1 complex with dissociation constants in the nanomolar range.
    • Binding of C1q to C1r2 and C1r2C1s2 zymogen complex shows no cooperativity.
    • C1q exhibits positive cooperativity when binding to C1s, with dissociation constants in the micromolar to nanomolar range.
    • No interaction was observed between C1q and the activated C1r2 complex.

    Conclusions:

    • The primary interaction between C1q and the C1 complex (C1r2C1s2) involves strong binding to the C1r2 subunit.
    • This critical interaction is disrupted upon activation of C1r2.
    • The findings shed light on the regulation of the classical complement pathway initiation.