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Related Experiment Videos

Protein kinase C and parietal cell function.

W Beil1, W Mannschedel, K F Sewing

  • 1Abteilung Allgemeine Pharmakologie, Medizinische Hochschule Hannover, F.R.G.

Biochemical and Biophysical Research Communications
|December 16, 1987
PubMed
Summary

12-O-tetradecanoylphorbol-13-acetate (TPA) reduces protein kinase C (PK-C) activity in guinea-pig parietal cells. TPA also inhibits gastric acid secretion by affecting the proton pump and H+ gradient.

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Area of Science:

  • Cell biology
  • Biochemistry
  • Physiology

Background:

  • Parietal cells are crucial for gastric acid secretion.
  • Protein kinase C (PK-C) plays a role in cellular signaling.
  • The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is a known activator of PK-C.

Purpose of the Study:

  • To investigate the effect of TPA on PK-C activity in guinea-pig parietal cells.
  • To determine how TPA influences hydrogen ion (H+) secretion and the gastric proton pump.

Main Methods:

  • Measurement of PK-C activity in cytosolic and particulate fractions of guinea-pig parietal cells.
  • Assessment of TPA's effect on H+ secretion in intact gastric vesicles.
  • Analysis of TPA's impact on the H+ gradient using a protonophoric action.

Main Results:

  • In resting cells, PK-C activity was equally distributed between cytosolic and particulate fractions.
  • TPA exposure led to a decrease in cytosolic PK-C activity without a corresponding increase in particulate activity.
  • TPA was found to inhibit the K+/H+-ATPase and dissipate the H+ gradient in gastric vesicles.

Conclusions:

  • TPA alters PK-C distribution and inhibits gastric acid secretion.
  • The antisecretory action of TPA involves direct inhibition of the K+/H+-ATPase and disruption of the H+ gradient.

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