Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fast ratiometric colorimetric sensing of total protein content in human serum based on classic biuret method.

Analytical and bioanalytical chemistry·2026

Same author

Highly fluorescent copper nanoclusters as programmable reporters for CRISPR/Cas12a-based detection of bacterial DNA.

Biosensors & bioelectronics·2026

Same author

Oropouche Fever: An imported case underscoring the importance of clinical and epidemiological vigilance.

Travel medicine and infectious disease·2025

Same author

Noncompetitive Determination of Small Analytes by Sandwich-Type Lateral Flow Assay Based on an Aptamer Kissing Complex.

Analytical chemistry·2024

Same author

Quantification of Ions and Organic Molecules, in Nanoliter Samples, in the Absence of Reference Materials.

Analytical chemistry·2022

Same author

Experiences of parents and caregivers in pediatric intensive care units: A qualitative study.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2022

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

A colorimetric nanosensor based on a selective target-responsive aptamer kissing complex.

E Goux¹, E Dausse², V Guieu¹

¹University Grenoble Alpes, DPM CNRS UMR 5063, F-38041 Grenoble, France. eric.peyrin@univ-grenoble-alpes.fr valerie.guieu@univ-grenoble-alpes.fr.

|March 10, 2017

Summary

This summary is machine-generated.

We developed a novel colorimetric aptasensor using gold nanoparticles. This sensor detects analytes via DNA and RNA hairpin interactions, causing a visible color change.

More Related Videos

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Mar 6, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Nanotechnology
Biochemistry
Analytical Chemistry

Background:

Colorimetric aptasensors offer sensitive detection methods.
Gold nanoparticles are widely used due to their unique optical properties.
Analyte detection often requires complex signal transduction.

Purpose of the Study:

To develop a novel colorimetric aptasensor.
To utilize functionalized gold nanoparticle probes for analyte detection.
To demonstrate a new mechanism for signal generation based on hairpin interactions.

Main Methods:

Conjugation of gold nanoparticles with two functional DNA and RNA hairpins.
Utilizing kissing (loop-loop) interactions between hairpins for analyte binding.
Observing color change from red to purple due to nanoparticle aggregation.

Main Results:

Successful design of a colorimetric aptasensor.
Demonstration of analyte-induced hairpin interactions.
Visible color change indicating successful detection and aggregation.

Conclusions:

The developed aptasensor provides a novel colorimetric detection method.
Kissing interactions of DNA/RNA hairpins offer a viable mechanism for aptasensor signal transduction.
This approach enables sensitive detection of small analyte ligands.