Related Experiment Video
Updated: Aug 2, 2026

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
Published on: October 2, 2012
Regulation of lactate/pyruvate ratios by cyclic AMP in Neurospora crassa
1Program in Genetics, Washington State University, Pullman 99164-4350.
Abstract:
Cyclic AMP is thought to have a general role in stimulating the breakdown of carbohydrate reserves and subsequent glycolytic activity. This would be expected to increase the availability of reducing equivalents in the form of cytoplasmic NADH. The current study examines another potential reaction controlling cytoplasmic NADH in the fungus Neurospora crassa, that of lactate dehydrogenase, to determine whether it is also regulated by cyclic AMP. The cr-1, adenylate cyclase and cyclic AMP-deficient mutant, grown with and without exogenous cyclic AMP was compared with an isogenic wild type. The results show that cyclic AMP raises pyruvic acid pools and lowers both lactic acid pools and lactate/pyruvate ratios. It does that, in part or in whole, by lowering lactate dehydrogenase activity. The possibility that cytoplasmic NAD+/NADH is a major target of cyclic AMP control is discussed. The high performance liquid chromatography procedures used in these studies are applicable to the measurement of intracellular pools of tricarboxylic acid cycle and other organic acids.
More Related Videos
09:35Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
Published on: October 15, 2018
07:18Visualizing Monocarboxylates and Other Relevant Metabolites in the Ex Vivo Drosophila Larval Brain Using Genetically Encoded Sensors
Published on: October 27, 2023
Related Concept Videos
Operons
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Inducible Operons: lac Operon
Gene Regulation in Microbial Communities: Quorum Sensing
Gene Regulation During Sporulation
Stringent Response in E. coli