Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Factors Affecting Erythropoiesis01:24

Factors Affecting Erythropoiesis

6.5K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
6.5K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

15.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
15.5K
Oxygen Transport in the Blood01:27

Oxygen Transport in the Blood

7.7K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
7.7K
Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

5.0K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Cytotoxicity of Ochratoxin A: Pro-Apoptotic Effects on Healthy Immune Cells Compared to Leukemia Cells.

Molecules (Basel, Switzerland)·2025
Same author

Pro-Apoptotic and Cytotoxic Effects of Melittin on HL-60 Acute Promyelocytic Leukemia Cells: Implications for Retinoid-Independent Therapy.

Molecules (Basel, Switzerland)·2025
Same author

Ricin and Abrin in Biosecurity: Detection Technologies and Strategic Responses.

Toxins·2025
Same author

Gut as a Target of Ochratoxin A: Toxicological Insights and the Role of Microbiota.

International journal of molecular sciences·2025
Same author

Evaluation of a Method for Assessing Food Contamination Based on a Shopping Mall Model.

Foods (Basel, Switzerland)·2025
Same author

Acetylcholinesterase as a Multifunctional Target in Amyloid-Driven Neurodegeneration: From Dual-Site Inhibitors to Anti-Agregation Strategies.

International journal of molecular sciences·2025
Same journal

Repurposing nitazoxanide as a novel ferroptosis inducer for triple-negative breast cancer via dual disruption of iron homeostasis and the β-catenin/GPX4 axis.

Redox report : communications in free radical research·2026
Same journal

HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors.

Redox report : communications in free radical research·2026
Same journal

Type III intermediate filaments as novel CoAlation targets.

Redox report : communications in free radical research·2026
Same journal

Ovalbumin oxidative modification fingerprints depend on gas plasma-driven reactive species profiles.

Redox report : communications in free radical research·2026
Same journal

Significance of GSH and H<sub>2</sub>S regulation for cancer: an intricate interplay between diet, microbiota, metabolic reprogramming, and immune health.

Redox report : communications in free radical research·2026
Same journal

Enzymatic and non-enzymatic oxidation of fibrillar collagen.

Redox report : communications in free radical research·2026
See all related articles

Related Experiment Video

Updated: Mar 6, 2026

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research
06:35

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Published on: March 29, 2024

1.3K

Vitamin C and Trolox decrease oxidative stress and hemolysis in cold-stored human red blood cells.

Kamila Czubak1, Adam Antosik1, Natalia Cichon1

  • 1a Department of General Biochemistry, Faculty of Biology and Environmental Protection , University of Lodz , Lodz , Poland.

Redox Report : Communications in Free Radical Research
|March 10, 2017
PubMed
Summary
This summary is machine-generated.

Sodium ascorbate alone did not prevent red blood cell damage during storage. However, combining sodium ascorbate with Trolox significantly protected red blood cells, reducing hemolysis and preserving membrane integrity.

Keywords:
Oxidative damageTroloxred blood cellsodium ascorbate (vitamin C)storage

More Related Videos

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

14.0K
Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
04:10

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

Published on: October 20, 2023

2.3K

Related Experiment Videos

Last Updated: Mar 6, 2026

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research
06:35

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Published on: March 29, 2024

1.3K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

14.0K
Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
04:10

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

Published on: October 20, 2023

2.3K

Area of Science:

  • Biochemistry
  • Hematology
  • Oxidative Stress Research

Background:

  • Red blood cells (RBCs) undergo oxidative damage during refrigerated storage, compromising their integrity and function.
  • Understanding the impact of antioxidants on RBC preservation is crucial for improving blood product viability.

Purpose of the Study:

  • To evaluate the efficacy of sodium ascorbate (SA) and a combination of SA and Trolox in mitigating oxidative stress in stored RBCs.
  • To assess the impact of these antioxidants on RBC membrane integrity and key oxidative markers.

Main Methods:

  • Human RBCs were stored under refrigeration for up to 20 days.
  • Hemolysis and lactate dehydrogenase (LDH) leakage were measured to assess membrane integrity.
  • Lipid peroxidation (LPO), glutathione (GSH), and total antioxidant capacity (TAC) were quantified.

Main Results:

  • SA alone did not significantly reduce storage-induced hemolysis or membrane permeability.
  • SA demonstrated a concentration-independent inhibition of LPO and increased TAC.
  • The combination of SA and Trolox significantly inhibited hemolysis, LDH leakage, LPO, and GSH depletion, while enhancing TAC.

Conclusions:

  • The pro- or antioxidant effects of vitamin C (SA) can be concentration-dependent and influenced by RBC degradation products during storage.
  • A combination of SA and Trolox shows potential as an effective additive for RBC storage, providing both antiradical and membrane-stabilizing protection.