Evidence for leucine-enkephalin immunoreactive neurons in the medulla which project to spinal cord in squirrel monkey

Rhodamine-labeled latex microsphere injections were combined with horseradish peroxidase immunohistochemistry in squirrel monkeys to reveal neurons in the medullary raphe and medial reticular formation which projected to spinal cord and were positive for leucine-enkephalin-like immunoreactivity. Double-labeled cells were found primarily in nucleus raphe magnus, the reticular nucleus magnocellularis, and the lateral reticular nucleus. These results provide evidence for a descending projection from enkephalin-containing cells of the rostral ventral medulla, a region which has been strongly implicated in antinociception.