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Updated: Mar 6, 2026

A Murine Model of Vertical Sleeve Gastrectomy
Published on: December 18, 2017
Sleeve gastrectomy drives persistent shifts in the gut microbiome
Cyrus Jahansouz1, Christopher Staley2, David A Bernlohr3
1Department of Surgery, University of Minnesota, Minneapolis, Minnesota.
Background:
Changes in the gut microbiome following bariatric surgery have been causally linked to metabolic benefits.
Objectives:
We sought to characterize and assess the stability of gut microbiome shifts following sleeve gastrectomy (SG).
Setting:
University laboratories.
Methods:
Diet-induced obese mice were randomized to SG or sham surgery. Mice were housed individually or cohoused such that one SG mouse was housed with one weight-matched, sham-operated mouse. Fecal samples were collected before and on postoperative days 7 and 28. Bacterial composition in feces was characterized by using next-generation Illumina sequencing of 16 S rRNA.
Results:
SG mice lost more weight and were more insulin sensitive than sham mice independent of housing status (P<.05). One week following surgery, fecal samples from all mice showed shifts in the microbiome that only persisted in SG-operated mice. Cohousing did not alter the microbial composition of SG-operated mice. Cohoused sham-operated mice showed a unique shift in microbial composition on postoperative day 28 that differed from individually housed, sham-operated mice (P<.001). Cohousing did not affect metabolic outcomes of either SG or sham surgeries.
Conclusion:
SG results in acute and sustained shifts in the gut microbiome. SG associated shifts are not altered by reexposure to obesity-associated gut microbiota.
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