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Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
Published on: December 31, 2014
In vitro BALB/3T3 cell transformation assay of nonoxynol-9 and 1,4-dioxane
C W Sheu1, F M Moreland, J K Lee
1Genetic Toxicology Branch, Food and Drug Administration, Washington, DC 20204.
Environmental and Molecular Mutagenesis
|January 1, 1988
Abstract:
The spermicidal surfactant nonoxynol-9 (Igepal CO-630, GAF Corp.) and a potential impurity, 1,4-dioxane, were tested in the in vitro cell transformation assay using BALB/3T3 cells. Two treatment periods, 48 hr and 13 days, were used. Nonoxynol-9, tested at levels up to 10 micrograms/ml, did not induce transformation, whereas dioxane was very active in the induction of type III foci in the cultured BALB/3T3 cells.
