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Quantifying Hormone Disruptors with an Engineered Bacterial Biosensor.

Ariel L Furst1, Alexander C Hoepker1, Matthew B Francis2

  • 1Department of Chemistry, University of California, Berkeley , Berkeley, California 94720, United States.

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|March 11, 2017
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Summary
This summary is machine-generated.

A new portable test detects endocrine disrupting compounds (EDCs) by measuring total estrogenic activity. This rapid, broad-spectrum method uses engineered bacteria to identify harmful chemicals in environmental and consumer products.

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Toxicology

Background:

  • Endocrine disrupting compounds (EDCs) are prevalent environmental contaminants from various sources like pesticides and plastics.
  • EDCs are linked to significant health issues including obesity, diabetes, and cancer.
  • Current detection methods for EDCs are limited due to their high potency, chemical diversity, and low concentrations, lacking broad-spectrum, rapid testing capabilities.

Purpose of the Study:

  • To develop a novel, rapid, portable, and user-friendly detection system for endocrine disrupting compounds.
  • To create a broad-spectrum assay that measures total estrogenic activity rather than individual chemicals.
  • To enable efficient monitoring of environmental contamination and identification of new EDCs.

Main Methods:

  • Engineered *Escherichia coli* expressing a native estrogen receptor construct on its surface.
  • Utilized impedance measurements detecting bacterial binding to a modified electrode for signal amplification.
  • Developed a system reporting total estrogenic activity based on receptor binding, not specific compound identification.

Main Results:

  • Achieved detection of sub-ppb levels of estradiol and ppm levels of bisphenol A in complex solutions.
  • Successfully detected estrogenic chemicals released from a microwaved plastic baby bottle.
  • Demonstrated the system's ability to quantify total estrogenic activity, reflecting the sample's overall endocrine-disrupting potential.

Conclusions:

  • The developed biosensor offers a fast, portable, and broad-spectrum method for detecting endocrine disrupting compounds.
  • This approach provides a reliable measure of total estrogenic activity, crucial for risk assessment.
  • The technology holds promise for widespread application in monitoring diverse chemical classes that interact with specific biological receptors.